Isolation and Identification of Active Compound of n-hexane Fraction from Kasturi (Mangifera casturi Konsterm.) against Antioxidant and Immunomodulatory Activity

Sutomo, Sutomo and Subagus, Wahyuono and Sugeng, Rianto and Erna , Prawita Setyowati (2013) Isolation and Identification of Active Compound of n-hexane Fraction from Kasturi (Mangifera casturi Konsterm.) against Antioxidant and Immunomodulatory Activity. Journal of Biological Sciences , 7 (13). pp. 596-604. ISSN 1727-3048

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Abstract

: A research had been conducted to isolated, to identify and to test biological activity of active isolates from n-hexane extracts of casturi (Mangifera casturi Konsterm.) fruit. The research aims at isolating active isolates from n-hexane fractions and testing for antioxidant and immunomodulatory activities. n-hexane fractions were isolated using column chromatographic method by using n-hexane-ethylacetate as eluents with eluent gradient (20:1; 15:1; 10:1; 8:2; 7:3; 6:4 and 5:5) v/v. The isolated compounds (purified isolates) were white crystals identified as terpenoid compound. Identification of chemical structures found a compound, namely, 1-isopropenyl-3a, 5a, 5b, 8, 8, 11a-hexamethyl-eicosahydro-cyclo penta [α]chrysen-9-ol (lupeol). The isolated compound was tested for antioxidant activity using DPPH (1,1-diphenyl -2-picrylhydrazyl) method with an IC50 value of 14384.71-lower than that of quercetin control (IC50 = 2.962). The finding shows that the isolate is not a potent compound against DPPH free radical inhibition. In vitro immunomodulatory activity test was conducted for the isolated compound. The isolates were given at dosages of 6.25, 12.5, 25, 50 and 100 μg. The test revealed increasing macrophage activity against latex bead phagocytosis. The highest phagocytosis activity and capacity were found at a dosage of 100 μg, namely, latex-bead consuming macrophage by 26% and macrophage-eaten latex bead by 32% (significant, compared to the negative control, namely, latex-bead eating macrophage by 13% and macrophage-eaten latex bead by 15%). Keywords : Mangifera casturi Kosterm, n-hexane fraction, lupeol, antioxidant, immuonomodulatory

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas MIPA > FARMASI
Depositing User: Mr Arief Mirathan - Eka Setya Wijaya
Date Deposited: 26 Nov 2015 02:46
Last Modified: 26 Nov 2015 02:46
URI: http://eprints.unlam.ac.id/id/eprint/542

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