AKTIVITAS ANTIBAKTERI DARI MELANIN TINTA SOTONG DAN CUMI-CUMI

FITRIAL, YUSPIHANA AKTIVITAS ANTIBAKTERI DARI MELANIN TINTA SOTONG DAN CUMI-CUMI. JPHPI.

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Official URL: http://journal.ipb.ac.id/index.php/jphpi/article/v...

Abstract

Abstrak Lingkungan laut terdiri atas banyak organisme yang diketahui memiliki senyawa bioaktif sebagai alat pertahanan diri atau untuk melindungi telur dan embrionya. Kelas Cephalopoda (seperti cumi-cumi dan sotong) terkenal karena pertahanan dirinya, seperti gerakan pelarian, perubahan warna, mengeluarkan racun dan tinta. Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri melanin dari tinta sotong (Sepia sp.) dengan tinta cumi-cumi (Loligo sp.) terhadap Escherichia coli. Ekstraksi dan pemurnian terhadap tinta sotong dan cumi-cumi dilakukan untuk mendapatkan melanin dengan menggunakan HCl 0,5 M secara mekanik. Melanin yang diperoleh diuji aktivitasnya terhadap E. coli dengan metode kontak langsung antara melanin dan E. coli di dalam nutrient broth. Total mikroba dihitung dengan metode hitungan cawan.Tinta yang berasal dari Sepia sp. ataupun Loligo sp. juga diuji aktivitasnya. Hasil penelitian menunjukkan bahwa melanin dari tinta sotong dan cumi-cumi memiliki aktivitas penghambatan pada konsentrasi 10 mg/mL dan 20 mg/mL, secara berturut-turut mencapai 99,99% terhadap E. coli. Tinta dari kedua jenis Cephalopoda tersebut pada konsentrasi yang sama dengan melanin,tidak menunjukkan adanya aktivitas penghambatan terhadap E. coli. Melanin dari Sepia sp. memiliki aktivitas antibakteri terhadap E. coli lebih tinggi dibandingkan melanin dari Loligo sp. Kata kunci: aktivitas antibakteri, E. coli, Loligo sp., melanin, tinta, Sepia sp. Antibacterial Activity of Melanin from Cuttlefish and Squid Ink Abstract Marine environment comprises of many organism which are known to posses bioactive compound as a common means of self-defense or for the protection of eggs and embryos. Class Cephalopods (such as squid and cuttlefish) are notable for their defences, such as jetting escape movements, changes in colouration, toxic venom and inking.This study aims to compare the antibacterial activity of melanin from cuttlefish ink (Sepia sp.) with squid ink (Loligo sp.) against E. coli. Extraction and purification studies were carried out on Sepia and Loligo melanin using a hydrochloric acid 0,5M treatment under mechanical.The melanins were obtained and further evaluated their activity by direct contact methods between melanin and E. coli in nutrient broth.Total microbes was counted by total plate count.Both inks also was tested their activity against E. coli. The results showed that melanin from cuttlefish and squid inks had inhibitory activity at concentrations of 10 mg / ml and 20 mg / ml, respectively reaching 99.99% against E. coli.The inks of both Cephalopods at the same concentration as melanin, did not show any inhibitory activity against E. coli. The melanin of Sepia sp. have a higher antibacterial activity than the melanin of Loligo sp. Keywords: antibacterial activity, cuttlefish, E. coli, Loligo sp., melanin, Sepia sp

Item Type: Article
Subjects: A General Works > AI Indexes (General)
Depositing User: Mr Arief Mirathan
Date Deposited: 06 Dec 2017 01:21
Last Modified: 06 Dec 2017 01:21
URI: http://eprints.ulm.ac.id/id/eprint/2541

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