Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa

Sulaiman, Abrani Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa. Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa. ISSN 0126 - 0421

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Official URL: https://jurnal.ugm.ac.id/jsv

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, since the spermatozoa is motile and has ability for fertilizing the oocyte. The objective of this research was to examine the effectivity of maltose in maintaining viability of ettawa crossbreed o goat epididymal spermatozoa preserved at 3–5 C. Five testis with epididymides of ettawa crossbreed goat were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa was divided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g maltose/100 ml (M0.3), and Tris extender + 0.6 g maltose/100 ml (M0.6), respectively. Dilutedo spermatozoa was stored in refrigerator at 3–5 C. Quality of diluted-spermatozoa including percentages of motile o spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3–5 C for four days. Data were analyzed using completely randomized design with three treatments and five replicates. Means were compared significant difference test at 0.05 significant level. Results of this study showed that mean spermatozoa concentration, percentage of MS, percentage of LS, and percentage of abnormal spermatozoa of ettawa crossbreed goat fresh epididymal spermatozoa were 3,220 million cell/ml, 70%, 81%, and 4.3%, respectively. At day-5 of storage, percentages of MS and LS for M0.3 (38 and 60.4%) and M0.6 (38 and 57.2%) were significantly (P<0.05) higher than control (32 and 55.4%). In conclusion, addition of 0.3 and 0.6% maltose in Tris o extender could be maintained viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5 C for three days. Key words: Maltose, preservation, epididymal spermatozoa, ettawa crossbreed goat.

Item Type: Article
Subjects: A General Works > AI Indexes (General)
Depositing User: Mr Arief Mirathan - Eka Setya Wijaya
Date Deposited: 14 Sep 2017 00:30
Last Modified: 14 Sep 2017 00:30
URI: http://eprints.unlam.ac.id/id/eprint/2185

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