Exploring The Scientific Evidences of The Uses of Carica papaya Leaves as Jamu and Vegetable and Establishing a Validated Quantification Method

Agung, Nugroho and Hesty , Heryani and Park, Hee-Juhn (2015) Exploring The Scientific Evidences of The Uses of Carica papaya Leaves as Jamu and Vegetable and Establishing a Validated Quantification Method. Project Report. Universitas Lambung Mangkurat . (Unpublished)

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29. Exploring The Scientific Evidences of The Uses of Carica papaya Leaves as Jamu and Vegetable and Establishing a Validated Quantification Method (First Year Program).pdf

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Abstract

In Indonesia, utilization of papaya leaf (Carica papaya L.) as a cooked vegetable and traditional herbal medicine (called jamu) is very common. Some of local community consume jamu of papaya leaf daily to keep their body fitness and prevent from some illness. Scientific investigation to deliver comprehensive evidences of the traditional and medicinal uses of papaya leaf is still not adequate, especially in the aspect of its biological active constituents. Antioxidative and vasodilatatory effects of methanol extract of papaya leaf that implicate in the reduction of cardiovascular risks have been demonstrated. This study was focused on the isolation and identification of the active constituents of methanol extract of C. papaya leaf. Variety of C. papaya used in this experiment was IPB9 that collected in Pelaihari, South Kalimantan. Chromatographic isolation was performed using several sizes of open columns with two different polarity of stationary phases, silica gel 60 (0.063-0.200 mm) and ODS (12 nm, S-75 μm), which was preceded by alcoholic extraction and fractionation process. Structures of the isolated compounds were determined by comparison of their physical (appearance, color, melting point) and spectroscopic data (UV-Vis, IR, 1H- and 13C-NMR) with those reported in the literatures. The eight isolated compounds were identified as gallic acid, caffeic acid, mauritianin, rutin, myricitrin, kaempferol rutinoside, quercetin, and kaempferol, which belonging to phenolic compounds. Quantification using an HPLC system showed that mauritianin (kaempferol 3-O-[-L-rhamnopyranosyl (12)][-L-rhamnopyranosyl (16)]--D-glucopyranoside) was the highest content of flavonoids in the MeOH extract (7.23 mg/g) and BuOH fraction (123.18 mg/g).

Item Type: Monograph (Project Report)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian > Teknologi Industri Pertanian
Depositing User: Mr Arief Mirathan - Eka Setya Wijaya
Date Deposited: 01 Jun 2017 02:49
Last Modified: 01 Jun 2017 02:49
URI: http://eprints.unlam.ac.id/id/eprint/1755

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