Effect of Antibitiotic Applications on Salivary Amylase and Catalase Kinetic Parameters on Neonatal at Risk of Sepsis In Vitro

Yunanto, Ari and Gunawan, Priscillia and Iskandar, Iskandar and Suhartono, Eko Effect of Antibitiotic Applications on Salivary Amylase and Catalase Kinetic Parameters on Neonatal at Risk of Sepsis In Vitro. Jurnal Internasional Bereputasi. ISSN 0975-5160

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Abstract

ABSTRACT In this present study, we try to demonstrate the effect of antibiotic applications to salivary amylase and catalase kinetic parameters on neonatal at risk of sepsis. This present study was performed at February-June 2015. Saliva samples were taken from 20 newborns(5 from normal newborn and 15 from infants were a risk of sepsis) treated in Ulin General Hospital, Banjarmasin, South Kalimantan, Indonesia. Saliva samples then divided into two groups, one group for salivary amylase and another group for salivary catalase kinetic parameter analysis, respectively. Each group will be divided into 4 subgroups with; T0 served as control which contains saliva+starch or H2O2, T1 which contains saliva+meropenem+starch or H2O2; T2 which contains saliva+amikacin+starch or H2O2; and T3 which contains saliva+diazole+starch or H2O2. Solutions then incubated at 37oC for 1 hours and then was prepared for kinetic parameter analysis. The kinetics parameters (Km and Vmax) were determined using Lineweaver-Burk plot. The results showed that antibiotic treatments could decrease the affinity of both amylum-amylase and H2O2-catalase complex which expressed by the higher Km and Vmax values. From this results, it can be concluded that antibiotic works not just by the common mechanisms, but work with another mechanism, ie. by decreased glucose and increased H2O2 concentrations that will have a negatif effect for bacterial living in neonatal at risk of sepsis.

Item Type: Article
Subjects: V Naval Science > V Naval Science (General)
Depositing User: Mr Arief Mirathan - Eka Setya Wijaya
Date Deposited: 21 May 2016 03:58
Last Modified: 21 May 2016 03:58
URI: http://eprints.unlam.ac.id/id/eprint/1037

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